Glycoside Hydrolase (GH) 45 and 5 Candidate Cellulases in Aphelenchoides besseyi Isolated from Bird’s-Nest Fern

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Glycoside Hydrolase (GH) 45 and 5 Candidate Cellulases in Aphelenchoides besseyi Isolated from Bird’s-Nest Fern

Five Aphelenchoides besseyi isolates collected from bird's-nest ferns or rice possess different parasitic capacities in bird's-nest fern. Two different glycoside hydrolase (GH) 45 genes were identified in the fern isolates, and only one was found in the rice isolates. A Abe GH5-1 gene containing an SCP-like family domain was found only in the fern isolates. Abe GH5-1 gene has five introns sugge...

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Currently, the cost of cellulase enzymes remains a key economic impediment to commercialization of biofuels. Enzymes from glycoside hydrolase family 48 (GH48) are a critical component of numerous natural lignocellulose-degrading systems. Although computational mining of large genomic data sets is a promising new approach for identifying novel cellulolytic activities, current computational metho...

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Defining Cellulase in the Glycosyl Hydrolase Family 48 Sequence, Structure, and Evolution of Cellulases in the Glycoside Hydrolase Family 48

Background: Cellulases are non-homologous isofunctional enzymes, which prevents their unambiguous identification in genomic datasets. Results: Cellulases from glycoside hydrolase family 48 have distinct evolutionarily conserved sequence and structural features. Conclusion: Conserved sequence/structure features can be used to differentiate cellulases from non-cellulases in genomic datasets. Sign...

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ژورنال

عنوان ژورنال: PLOS ONE

سال: 2016

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0158663